PrimePCR™ SYBR® Green Assay: ZBTB38, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024118

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZBTB38, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZBTB38, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001638
Assay Design:   Exonic
Chromosome Location:   3:141161317-141161450question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000509842 ENST00000507722 ENST00000513258 ENST00000509883 ENST00000514251 ENST00000510338 ENST00000504673 ENST00000513570 ENST00000441582 ENST00000510726 ENST00000509813 ENST00000321464

Gene Information

The protein encoded by this gene is a zinc finger transcriptional activator that binds methylated DNA. The encoded protein can form homodimers or heterodimers through the zinc finger domains. In mouse inhibition of this protein has been associated with apoptosis in some cell types. [provided by RefSeq Jun 2010]

Gene Symbol:   ZBTB38
Gene Name:   zinc finger and BTB domain containing 38
Aliases:   CIBZ, FLJ22332, FLJ31131, FLJ35036, ZNF921
RefSeq:   NC_000003.11 NG_021426.1 NT_005612.16
Ensembl:   ENSG00000177311
Entrez:   253461
Chromosome Mapping:   3q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.890000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download