PrimePCR™ SYBR® Green Assay: GPR55, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024136

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GPR55, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPR55, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001676
Assay Design:   Exonic
Chromosome Location:   2:231775345-231775513question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000392039 ENST00000438398 ENST00000392040

Gene Information

Members of the G protein-coupled receptor (GPR) family such as GPR55 play important roles in signal transduction from the external environment to the inside of the cell (Sawzdargo et al. 1999 [PubMed 9931487]).[supplied by OMIM Apr 2008]

Gene Symbol:   GPR55
Gene Name:   G protein-coupled receptor 55
Aliases:   Not Available
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000135898
Entrez:   9290
Chromosome Mapping:   2q37

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.100000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download