PrimePCR™ SYBR® Green Assay: GPBAR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GPBAR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPBAR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001871
Assay Design:   Exonic
Chromosome Location:   2:219128416-219128509question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000479077 ENST00000522678 ENST00000519574 ENST00000521462

Gene Information

This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP activation of a MAP kinase signaling pathway and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   GPBAR1
Gene Name:   G protein-coupled bile acid receptor 1
Aliases:   BG37, GPCR19, GPR131, M-BAR, MGC40597, TGR5
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000179921
Entrez:   151306
Chromosome Mapping:   2q35

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