PrimePCR™ SYBR® Green Assay: TRAF4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024271

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRAF4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRAF4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002086
Assay Design:   Exonic
Chromosome Location:   17:27075074-27075184question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000262395 ENST00000422344 ENST00000444415 ENST00000454852 ENST00000262396

Gene Information

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor p75 (NTR/NTSR1) and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TRAF4
Gene Name:   TNF receptor-associated factor 4
Aliases:   CART1, MLN62, RNF83
RefSeq:   NC_000017.10 NG_012263.1 NT_010799.15
Ensembl:   ENSG00000076604
Entrez:   9618
Chromosome Mapping:   17q11-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.890000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download