PrimePCR™ SYBR® Green Assay: CDK16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0024275


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PrimePCR™ PreAmp for SYBR® Green Assay: CDK16, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CDK16, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0002106
Assay Design:		Exonic
Chromosome Location:		X:47083784-47083892question
Amplicon Length:		79
Splice Variants Targeted:		ENST00000457458 ENST00000357227 ENST00000519758 ENST00000520893 ENST00000517426 ENST00000518391 ENST00000518022 ENST00000523034 ENST00000540877 ENST00000276052 ENST00000540311

Gene Information

The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Dec 2009]

Gene Symbol:		CDK16
Gene Name:		cyclin-dependent kinase 16
Aliases:		FLJ16665, PCTAIRE, PCTAIRE1, PCTGAIRE, PCTK1
RefSeq:		NC_000023.10 NG_012546.1 NT_079573.4 NG_012517.1
Ensembl:		ENSG00000102225
Entrez:		5127
Chromosome Mapping:		Xp11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.340000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.