This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0024284
PrimePCR™ PreAmp for SYBR® Green Assay: ARHGDIA, Human
PrimePCR™ Template for SYBR® Green Assay: ARHGDIA, Human
Aplysia Ras-related homologs (ARHs) also called Rho genes belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive GDP-bound state by interaction with GDP dissociation inhibitors such as ARHGDIA (Leffers et al. 1993 [PubMed 8262133]).[supplied by OMIM Jan 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.