PrimePCR™ SYBR® Green Assay: MCHR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024309

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MCHR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MCHR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002205
Assay Design:   Exonic
Chromosome Location:   22:41077222-41077337question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000249016

Gene Information

The protein encoded by this gene a member of the G protein-coupled receptor family 1 is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors this protein does not seem to bind somatostatin. [provided by RefSeq Jul 2008]

Gene Symbol:   MCHR1
Gene Name:   melanin-concentrating hormone receptor 1
Aliases:   GPR24, MCH1R, MGC32129, SLC1
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000128285
Entrez:   2847
Chromosome Mapping:   22q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.420000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download