PrimePCR™ SYBR® Green Assay: TSSC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024311

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TSSC4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TSSC4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002212
Assay Design:   Exonic
Chromosome Location:   11:2423921-2424028question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000333256 ENST00000437110 ENST00000435795 ENST00000485682 ENST00000496468 ENST00000451491

Gene Information

This gene is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocortical carcinoma and lung ovarian and breast cancer. This gene is located among several imprinted genes; however this gene as well as the pan-hematopoietic expression gene (PHEMX) escapes imprinting. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq Jul 2008]

Gene Symbol:   TSSC4
Gene Name:   tumor suppressing subtransferable candidate 4
Aliases:   Not Available
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000184281
Entrez:   10078
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.130000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download