PrimePCR™ SYBR® Green Assay: PPIP5K1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024319

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPIP5K1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPIP5K1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002240
Assay Design:   Exonic
Chromosome Location:   15:43826083-43826247question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000381885 ENST00000360301 ENST00000360135 ENST00000334933 ENST00000396923 ENST00000381879 ENST00000304953 ENST00000381878 ENST00000420765 ENST00000348806

Gene Information

This gene encodes a dual functional inositol kinase. The encoded enzyme converts inositol hexakisphosphate to diphosphoinositol pentakisphosphate and diphosphoinositol pentakisphosphate to bis-diphosphoinositol tetrakisphosphate. This protein may be important for intracellular signaling pathways. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 15.[provided by RefSeq Jun 2010]

Gene Symbol:   PPIP5K1
Gene Name:   diphosphoinositol pentakisphosphate kinase 1
Aliases:   DKFZp313L0221, HISPPD2A, IP6K, IPS1, KIAA0377, MGC51871, VIP1, hsVIP1
RefSeq:   NC_000015.9 NG_005156.1 NG_022929.1 NT_010194.17
Ensembl:   ENSG00000168781
Entrez:   9677
Chromosome Mapping:   15q15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.520000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download