PrimePCR™ SYBR® Green Assay: AP3M2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024323

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AP3M2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AP3M2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002253
Assay Design:   Exonic
Chromosome Location:   8:42022947-42024689question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000518421 ENST00000396926 ENST00000521280 ENST00000517922 ENST00000517499 ENST00000174653

Gene Information

This gene encodes a subunit of the heterotetrameric adaptor-related protein comlex 3 (AP-3) which belongs to the adaptor complexes medium subunits family. The AP-3 complex plays a role in protein trafficking to lysosomes and specialized organelles. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Aug 2008]

Gene Symbol:   AP3M2
Gene Name:   adaptor-related protein complex 3, mu 2 subunit
Aliases:   AP47B, CLA20, P47B
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000070718
Entrez:   10947
Chromosome Mapping:   8p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.740000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download