PrimePCR™ SYBR® Green Assay: UPP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024339

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: UPP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: UPP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002297
Assay Design:   Exonic
Chromosome Location:   7:48147000-48147098question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000331803 ENST00000395564 ENST00000341253 ENST00000429491

Gene Information

The 2 known types of pyrimidine nucleoside phosphorylases uridine phosphorylase (UP; EC 2.4.2.3) and thymidine phosphorylase (TP; EC 2.4.2.4) in the presence of orthophosphate catalyze the reversible phosphorolysis of uridine and thymidine or deoxyuridine respectively to free bases and ribose-1-phosphate or deoxyribose-1-phosphate. Pyrimidine nucleoside phosphorylases can add ribose or deoxyribose to pyrimidine bases to form nucleosides that can be incorporated into RNA or DNA (Watanabe and Uchida 1995 [PubMed 7488099]).[supplied by OMIM Aug 2009]

Gene Symbol:   UPP1
Gene Name:   uridine phosphorylase 1
Aliases:   UDRPASE, UP, UPASE, UPP
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000183696
Entrez:   7378
Chromosome Mapping:   7p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.700000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download