PrimePCR™ SYBR® Green Assay: SLC39A14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: SLC39A14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC39A14, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002314
Assay Design:   Exonic
Chromosome Location:   8:22262274-22262377question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000359741 ENST00000520644 ENST00000240095 ENST00000381237 ENST00000289952 ENST00000524285 ENST00000520832 ENST00000519960 ENST00000522881 ENST00000517552

Gene Information

Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein nucleic acid carbohydrate and lipid metabolism as well as in the control of gene transcription growth development and differentiation. SLC39A14 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson 2003 [PubMed 12659941]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC39A14
Gene Name:   solute carrier family 39 (zinc transporter), member 14
Aliases:   KIAA0062, LZT-Hs4, NET34, ZIP14, cig19
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000104635
Entrez:   23516
Chromosome Mapping:   8p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.270000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download