PrimePCR™ SYBR® Green Assay: SMARCD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0024433


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PrimePCR™ PreAmp for SYBR® Green Assay: SMARCD3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SMARCD3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0002584
Assay Design:		Exonic
Chromosome Location:		7:150937198-150937287question
Amplicon Length:		60
Splice Variants Targeted:		ENST00000262188 ENST00000392811 ENST00000356800 ENST00000347683

Gene Information

The protein encoded by this gene is a member of the SWI/SNF family of proteins whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Multiple alternatively spliced transcript variants have been found for this gene but the biological validity of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:		SMARCD3
Gene Name:		SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3
Aliases:		BAF60C, CRACD3, MGC111010, Rsc6p
RefSeq:		NC_000007.13 NT_007914.15
Ensembl:		ENSG00000082014
Entrez:		6604
Chromosome Mapping:		7q35-q36

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.430000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.