This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Sequence and coordinates from the reference genome around your assays amplicon.
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: BCAR1, Human
PrimePCR™ Template for SYBR® Green Assay: BCAR1, Human
BCAR1 or CAS is an Src (MIM 190090) family kinase substrate involved in various cellular events including migration survival transformation and invasion (Sawada et al. 2006 [PubMed 17129785]).[supplied by OMIM May 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.