PrimePCR™ SYBR® Green Assay: SLC35B2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024637

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC35B2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC35B2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003243
Assay Design:   Exonic
Chromosome Location:   6:44223136-44223301question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000393810 ENST00000393812 ENST00000341553 ENST00000538577 ENST00000537814

Gene Information

Sulfotransferases (e.g. SULT4A1; MIM 608359) use an activated form of sulfate 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) as a common sulfate donor for sulfation of glycoproteins proteoglycans and glycolipids in the endoplasmic reticulum and Golgi apparatus. SLC35B2 is located in the microsomal membrane and transports PAPS from the cytosol where it is synthesized into the Golgi lumen (Kamiyama et al. 2003 [PubMed 12716889]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC35B2
Gene Name:   solute carrier family 35, member B2
Aliases:   PAPST1, SLL, UGTrel4
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000157593
Entrez:   347734
Chromosome Mapping:   6p12.1-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.920000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download