PrimePCR™ SYBR® Green Assay: AKR1B10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best efficiency


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PrimePCR™ PreAmp for SYBR® Green Assay: AKR1B10, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: AKR1B10, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0003967
Assay Design:		Exonic
Chromosome Location:		7:134226024-134226144question
Amplicon Length:		91
Splice Variants Targeted:		ENST00000359579

Gene Information

This gene encodes a member of the aldo/keto reductase superfamily which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes and it is less active on hexoses. It is highly expressed in adrenal gland small intestine and colon and may play an important role in liver carcinogenesis. [provided by RefSeq Jul 2008]

Gene Symbol:		AKR1B10
Gene Name:		aldo-keto reductase family 1, member B10 (aldose reductase)
Aliases:		AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI, MGC14103
RefSeq:		NC_000007.13 NT_007933.15
Ensembl:		ENSG00000198074
Entrez:		57016
Chromosome Mapping:		7q33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.480000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.