PrimePCR™ SYBR® Green Assay: LTBP4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024931

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LTBP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LTBP4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004130
Assay Design:   Exonic
Chromosome Location:   19:41119822-41119921question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000204005 ENST00000308370 ENST00000396819 ENST00000545697 ENST00000243562

Gene Information

The protein encoded by this gene binds transforming growth factor beta (TGFB) as it is secreted and targeted to the extracellular matrix. TGFB is biologically latent after secretion and insertion into the extracellular matrix and sheds TGFB and other proteins upon activation. Defects in this gene may be a cause of cutis laxa and severe pulmonary gastrointestinal and urinary abnormalities. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq May 2010]

Gene Symbol:   LTBP4
Gene Name:   latent transforming growth factor beta binding protein 4
Aliases:   FLJ46318, FLJ90018, LTBP-4, LTBP4L, LTBP4S
RefSeq:   NC_000019.9 NT_011109.16 NG_021201.1
Ensembl:   ENSG00000090006
Entrez:   8425
Chromosome Mapping:   19q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.400000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download