PrimePCR™ SYBR® Green Assay: PDE6B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PDE6B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PDE6B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004220
Assay Design:   Exonic
Chromosome Location:   4:657559-657904question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000255622 ENST00000496514 ENST00000429163 ENST00000471824

Gene Information

Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE) resulting in the rapid hydrolysis of cGMP closure of cGMP-gated cation channels and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha beta and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2009]

Gene Symbol:   PDE6B
Gene Name:   phosphodiesterase 6B, cGMP-specific, rod, beta
Aliases:   CSNB3, CSNBAD2, PDEB, RP40, rd1
RefSeq:   NC_000004.11 NG_009839.1 NT_037622.5
Ensembl:   ENSG00000133256
Entrez:   5158
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.810000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download