PrimePCR™ SYBR® Green Assay: EEF1E1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: EEF1E1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EEF1E1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0004229
Assay Design:		Exonic
Chromosome Location:		6:8097552-8097665question
Amplicon Length:		84
Splice Variants Targeted:		ENST00000429723 ENST00000502429 ENST00000379715 ENST00000507463 ENST00000488226

Gene Information

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq Dec 2010]

Gene Symbol:		EEF1E1
Gene Name:		eukaryotic translation elongation factor 1 epsilon 1
Aliases:		AIMP3, P18
RefSeq:		NC_000006.11 NT_007592.15
Ensembl:		ENSG00000124802
Entrez:		9521
Chromosome Mapping:		6p24.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997800
y-intercept	35.330000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.