PrimePCR™ SYBR® Green Assay: TLR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004300
Assay Design:   Exonic
Chromosome Location:   4:38800387-38800512question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000502213 ENST00000505940 ENST00000515861 ENST00000506146 ENST00000508364 ENST00000308979

Gene Information

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site and/or from alternative splicing have been noted for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   TLR1
Gene Name:   toll-like receptor 1
Aliases:   CD281, DKFZp547I0610, DKFZp564I0682, KIAA0012, MGC104956, MGC126311, MGC126312, TIL, TIL. LPRS5, rsc786
RefSeq:   NC_000004.11 NG_016228.1 NT_016297.16
Ensembl:   ENSG00000174125
Entrez:   7096
Chromosome Mapping:   4p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.890000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download