PrimePCR™ SYBR® Green Assay: LAIR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LAIR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LAIR2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004467
Assay Design:   Exonic
Chromosome Location:   19:55019216-55019319question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000412608 ENST00000452094 ENST00000301202 ENST00000351841

Gene Information

The protein encoded by this gene is a member of the immunoglobulin superfamily. It was identified by its similarity to LAIR1 an inhibitory receptor present on mononuclear leukocytes. This gene maps to a region of 19q13.4 termed the leukocyte receptor cluster which contains 29 genes in the immunoglobulin superfamily including LAIR1. The function of this protein is unknown although it is thought to be secreted and may help modulate mucosal tolerance. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LAIR2
Gene Name:   leukocyte-associated immunoglobulin-like receptor 2
Aliases:   CD306, MGC71634
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000167618
Entrez:   3904
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.340000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download