PrimePCR™ SYBR® Green Assay: SUV39H1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0025104


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PrimePCR™ PreAmp for SYBR® Green Assay: SUV39H1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SUV39H1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0004652
Assay Design:		Exonic
Chromosome Location:		X:48566025-48566129question
Amplicon Length:		75
Splice Variants Targeted:		ENST00000376687 ENST00000337852 ENST00000448548 ENST00000453214

Gene Information

This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase methylating Lys-9 of histone H3. Overall it plays a vital role in heterochromatin organization chromosome segregation and mitotic progression. [provided by RefSeq Jul 2008]

Gene Symbol:		SUV39H1
Gene Name:		suppressor of variegation 3-9 homolog 1 (Drosophila)
Aliases:		KMT1A, MG44, SUV39H
RefSeq:		NC_000023.10 NG_012530.1 NT_079573.4
Ensembl:		ENSG00000101945
Entrez:		6839
Chromosome Mapping:		Xp11.23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.280000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.