PrimePCR™ SYBR® Green Assay: MTHFD1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0025186


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PrimePCR™ PreAmp for SYBR® Green Assay: MTHFD1L, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MTHFD1L, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0004881
Assay Design:		Exonic
Chromosome Location:		6:151209013-151226791question
Amplicon Length:		80
Splice Variants Targeted:		ENST00000367321 ENST00000367308 ENST00000443074 ENST00000425276 ENST00000367307 ENST00000392243

Gene Information

The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jun 2011]

Gene Symbol:		MTHFD1L
Gene Name:		methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Aliases:		DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, dJ292B18.2
RefSeq:		NC_000006.11 NT_025741.15
Ensembl:		ENSG00000120254
Entrez:		25902
Chromosome Mapping:		6q25.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	34.890000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.