PrimePCR™ SYBR® Green Assay: SLC2A14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $179.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: SLC2A14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC2A14, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004911
Assay Design:   Exonic
Chromosome Location:   12:8022459-8023466question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000340749 ENST00000543909 ENST00000431042 ENST00000396589 ENST00000535295 ENST00000546234 ENST00000542782 ENST00000535344 ENST00000537557 ENST00000535266 ENST00000542916 ENST00000535383 ENST00000535587 ENST00000539234

Gene Information

Members of the glucose transporter (GLUT) family including SLC2A14 are highly conserved integral membrane proteins that transport hexoses such as glucose and fructose into all mammalian cells. GLUTs show tissue and cell-type specific expression (Wu and Freeze 2002 [PubMed 12504846]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC2A14
Gene Name:   solute carrier family 2 (facilitated glucose transporter), member 14
Aliases:   DKFZp564K1672, GLUT14, SLC2A3P3
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000173262
Entrez:   144195
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.460000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download