PrimePCR™ SYBR® Green Assay: CAST, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CAST, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CAST, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004924
Assay Design:   Exonic
Chromosome Location:   5:96084222-96086308question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000510500 ENST00000437034 ENST00000508579 ENST00000509259 ENST00000515663 ENST00000395813 ENST00000359176 ENST00000325674 ENST00000504465 ENST00000509903 ENST00000511782 ENST00000510156 ENST00000511049 ENST00000309190 ENST00000341926 ENST00000508608 ENST00000395812 ENST00000510756 ENST00000508830 ENST00000338252

Gene Information

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4) and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jun 2010]

Gene Symbol:   CAST
Gene Name:   calpastatin
Aliases:   BS-17, MGC9402
RefSeq:   NC_000005.9 NG_027839.1 NT_034772.6
Ensembl:   ENSG00000153113
Entrez:   831
Chromosome Mapping:   5q15

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