PrimePCR™ SYBR® Green Assay: OSR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0025250


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PrimePCR™ PreAmp for SYBR® Green Assay: OSR2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: OSR2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0005028
Assay Design:		Exonic
Chromosome Location:		8:99961067-99961280question
Amplicon Length:		184
Splice Variants Targeted:		ENST00000523368 ENST00000297565 ENST00000435298 ENST00000522510 ENST00000520951 ENST00000518199 ENST00000457907

Gene Information

OSR2 is a mammalian homolog of the Drosophila odd-skipped family of transcription factors (Lan et al. 2004 [PubMed 15175245]).[supplied by OMIM Mar 2008]

Gene Symbol:		OSR2
Gene Name:		odd-skipped related 2 (Drosophila)
Aliases:		FLJ90037
RefSeq:		NC_000008.10 NT_008046.16
Ensembl:		ENSG00000164920
Entrez:		116039
Chromosome Mapping:		8q22.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.080000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.