PrimePCR™ SYBR® Green Assay: COL9A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0025318


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PrimePCR™ PreAmp for SYBR® Green Assay: COL9A2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: COL9A2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0005219
Assay Design:		Exonic
Chromosome Location:		1:40766412-40766615question
Amplicon Length:		174
Splice Variants Targeted:		ENST00000372748

Gene Information

This gene encodes one of the three alpha chains of type IX collagen the major collagen component of hyaline cartilage. Type IX collagen a heterotrimeric molecule is usually found in tissues containing type II collagen a fibrillar collagen. This chain is unusual in that unlike the other two type IX alpha chains it contains a covalently attached glycosaminoglycan side chain. Mutations in this gene are associated with multiple epiphyseal dysplasia. [provided by RefSeq Jul 2008]

Gene Symbol:		COL9A2
Gene Name:		collagen, type IX, alpha 2
Aliases:		DJ39G22.4, EDM2, MED
RefSeq:		NC_000001.10 NG_008031.1 NT_032977.9
Ensembl:		ENSG00000049089
Entrez:		1298
Chromosome Mapping:		1p33-p32

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	34.690000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.