PrimePCR™ SYBR® Green Assay: ST3GAL6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: ST3GAL6, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ST3GAL6, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0005310
Assay Design:		Exonic
Chromosome Location:		3:98512914-98513141question
Amplicon Length:		198
Splice Variants Targeted:		ENST00000483910 ENST00000394162 ENST00000265261

Gene Information

Sialyltransferases such as ST3GAL6 catalyze the transfer of sialic acid from cytidine 5-prime monophospho-N-acetylneuraminic acid (CMP-NeuAc) to terminal positions of glycoprotein and glycolipid carbohydrate groups. Terminal NeuAc residues are key determinants of carbohydrate structures such as the sialyl-Lewis X determinants and are widely distributed in many cell types.[supplied by OMIM Mar 2008]

Gene Symbol:		ST3GAL6
Gene Name:		ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Aliases:		SIAT10, ST3GALVI
RefSeq:		NC_000003.11 NT_005612.16
Ensembl:		ENSG00000064225
Entrez:		10402
Chromosome Mapping:		3q12.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.996200
y-intercept	33.850000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.