PrimePCR™ SYBR® Green Assay: IMPA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: IMPA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IMPA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005465
Assay Design:   Exonic
Chromosome Location:   8:82571077-82571290question
Amplicon Length:   184
Splice Variants Targeted:   ENST00000256108

Gene Information

This gene encodes an enyzme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol a precursor of phosphatidylinositol and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 145-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-13-diphosphate myo-inositol-14-diphosphate scyllo-inositol-phosphate glucose-1-phosphate glucose-6-phosphate fructose-1-phosphate beta-glycerophosphate and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq Nov 2009]

Gene Symbol:   IMPA1
Gene Name:   inositol(myo)-1(or 4)-monophosphatase 1
Aliases:   IMP, IMPA
RefSeq:   NC_000008.10 NG_015829.1 NT_008183.19
Ensembl:   ENSG00000133731
Entrez:   3612
Chromosome Mapping:   8q21.13-q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.780000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download