PrimePCR™ SYBR® Green Assay: PSG2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $179.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: PSG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PSG2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005826
Assay Design:   Exonic
Chromosome Location:   19:43568508-43568664question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000406487 ENST00000329509 ENST00000401942 ENST00000406917

Gene Information

The human pregnancy-specific glycoproteins (PSGs) are a family of proteins that are synthesized in large amounts by placental trophoblasts and released into the maternal circulation during pregnancy. Molecular cloning and analysis of several PSG genes has indicated that the PSGs form a subgroup of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily of genes. Members of the CEA family consist of a single N domain with structural similarity to the immunoglobulin variable domains followed by a variable number of immunoglobulin constant-like A and/or B domains. Most PSGs have an arg-gly-asp (RGD) motif which has been shown to function as an adhesion recognition signal for several integrins in the N-terminal domain (summary by Teglund et al. 1994 [PubMed 7851896]). For additional general information about the PSG gene family see PSG1 (MIM 176390).[supplied by OMIM Oct 2009]

Gene Symbol:   PSG2
Gene Name:   pregnancy specific beta-1-glycoprotein 2
Aliases:   CEA, PSBG2, PSG1, PSGGB
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000242221
Entrez:   5670
Chromosome Mapping:   19q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.590000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download