PrimePCR™ SYBR® Green Assay: EED, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: EED, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EED, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0006871
Assay Design:		Exonic
Chromosome Location:		11:85988032-85988124question
Amplicon Length:		63
Splice Variants Targeted:		ENST00000263360 ENST00000528180 ENST00000327320 ENST00000534595 ENST00000534564 ENST00000524673 ENST00000527888 ENST00000351625

Gene Information

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2 the cytoplasmic tail of integrin beta7 immunodeficiency virus type 1 (HIV-1) MA protein and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		EED
Gene Name:		embryonic ectoderm development
Aliases:		HEED, WAIT1
RefSeq:		NC_000011.9 NT_167190.1
Ensembl:		ENSG00000074266
Entrez:		8726
Chromosome Mapping:		11q14.2-q22.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.060000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.