PrimePCR™ SYBR® Green Assay: IRF7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0025658


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PrimePCR™ PreAmp for SYBR® Green Assay: IRF7, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IRF7, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0007783
Assay Design:		Exonic
Chromosome Location:		11:612632-612766question
Amplicon Length:		105
Splice Variants Targeted:		ENST00000397562 ENST00000525445 ENST00000348655 ENST00000397574 ENST00000330243 ENST00000397570 ENST00000397566

Gene Information

IRF7 encodes interferon regulatory factor 7 a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified although the functional consequences of these have not yet been established. [provided by RefSeq Jul 2008]

Gene Symbol:		IRF7
Gene Name:		interferon regulatory factor 7
Aliases:		IRF-7H, IRF7A
RefSeq:		NC_000011.9 NT_009237.18
Ensembl:		ENSG00000185507
Entrez:		3665
Chromosome Mapping:		11p15.5

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	35.950000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.