PrimePCR™ SYBR® Green Assay: MRPL20, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0032767

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MRPL20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MRPL20, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0020493
Assay Design:   Exonic
Chromosome Location:   1:1340767-1340910question
Amplicon Length:   114

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. [provided by RefSeq Jul 2008]

Gene Symbol:   MRPL20
Gene Name:   mitochondrial ribosomal protein L20
Aliases:   L20mt, MGC4779, MGC74465, MRP-L20
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000242485
Entrez:   55052
Chromosome Mapping:   1p36.3-p36.2

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