PrimePCR™ SYBR® Green Assay: PPCS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: PPCS, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PPCS, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0022282
Assay Design:		Exonic
Chromosome Location:		1:42925403-42925578question
Amplicon Length:		146
Splice Variants Targeted:		ENST00000372562 ENST00000372561 ENST00000372556 ENST00000455780

Gene Information

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5) one of the last enzymes in this pathway converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al. 2002 [PubMed 11923312]).[supplied by OMIM Mar 2008]

Gene Symbol:		PPCS
Gene Name:		phosphopantothenoylcysteine synthetase
Aliases:		FLJ11838, MGC117357, MGC138220
RefSeq:		NC_000001.10 NT_032977.9
Ensembl:		ENSG00000127125
Entrez:		79717
Chromosome Mapping:		1p34.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	34.650000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.