PrimePCR™ SYBR® Green Assay: IAPP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
Add to My PrimePCR Add to My PrimePCR		Add to Quote Add to Quote
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: IAPP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

List Price:		$174.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

PrimePCR™ Template for SYBR® Green Assay: IAPP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

List Price:		$179.00
Your Price:		Log In
Quantity:		Add to Cart Add to Cart
[ Add to My PrimePCR ] [ Add to My PrimePCR ]		[ Add to Quote ] [ Add to Quote ]

Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0023249
Assay Design:		Exonic
Chromosome Location:		12:21531421-21531510question
Amplicon Length:		60
Splice Variants Targeted:		ENST00000240652 ENST00000542023 ENST00000535428

Gene Information

Islet or insulinoma amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time a direct causative role for amylin has been harder to establish. Studies suggest that amylin like the related beta-amyloid (Abeta) associated with Alzheimer's disease can induce apoptotic cell-death in particular cultured cells an effect that may be relevant to the development of type II diabetes. [provided by RefSeq Apr 2011]

Gene Symbol:		IAPP
Gene Name:		islet amyloid polypeptide
Aliases:		DAP, IAP
RefSeq:		NC_000012.11 NT_009714.17
Ensembl:		ENSG00000121351
Entrez:		3375
Chromosome Mapping:		12p12.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.996000
y-intercept	36.140000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.