PrimePCR™ SYBR® Green Assay: UBC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0033881


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PrimePCR™ PreAmp for SYBR® Green Assay: UBC, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: UBC, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0023867
Assay Design:		Exonic
Chromosome Location:		12:125397941-125398089question
Amplicon Length:		119
Splice Variants Targeted:		ENST00000536769 ENST00000535460 ENST00000538617 ENST00000541046 ENST00000339647 ENST00000540351 ENST00000541645 ENST00000535131 ENST00000546271 ENST00000540700 ENST00000535859 ENST00000546120

Gene Information

This gene represents a ubiquitin gene ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation DNA repair cell cycle regulation kinase modification endocytosis and regulation of other cell signaling pathways. [provided by RefSeq Aug 2010]

Gene Symbol:		UBC
Gene Name:		ubiquitin C
Aliases:		HMG20
RefSeq:		NC_000012.11 NG_027722.1 NT_009755.19
Ensembl:		ENSG00000150991
Entrez:		7316
Chromosome Mapping:		12q24.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.994700
y-intercept	34.110000
Efficiency	108

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.