PrimePCR™ SYBR® Green Assay: ARL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ARL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0033984
Assay Design:   Exonic
Chromosome Location:   12:101794840-101794938question
Amplicon Length:   69

Gene Information

The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins which are structurally related to ADP-ribosylation factors (ARFs). ARFs described as activators of cholera toxin (CT) ADP-ribosyltransferase activity regulate intracellular vesicular membrane trafficking and stimulate a phospholipase D (PLD) isoform. Although ARL proteins were initially thought not to activate CT or PLD later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. [provided by RefSeq Jul 2008]

Gene Symbol:   ARL1
Gene Name:   ADP-ribosylation factor-like 1
Aliases:   ARFL1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000120805
Entrez:   400
Chromosome Mapping:   12q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.680000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download