PrimePCR™ SYBR® Green Assay: CBLL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039116

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CBLL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CBLL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036392
Assay Design:   exonic
Chromosome Location:   7:107398608-107398741question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000440859 ENST00000222597 ENST00000415884 ENST00000420796

Gene Information

This gene encodes an E3 ubiquitin-ligase for the E-cadherin complex and mediates its ubiquitination endocytosis and degradation in the lysosomes. The encoded protein contains a RING-finger domain and is also thought to have a role in control of cell proliferation. A related pseudogene has been identified on chromosome X. Alternative splicing results in a non-coding transcript variant. [provided by RefSeq Aug 2011]

Gene Symbol:   CBLL1
Gene Name:   Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
Aliases:   FLJ23109, HAKAI, MGC163401, MGC163403, RNF188
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000105879
Entrez:   79872
Chromosome Mapping:   7q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.080000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download