PrimePCR™ SYBR® Green Assay: CRB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039274

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CRB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CRB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036550
Assay Design:   exonic
Chromosome Location:   1:197390160-197390289question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000367400 ENST00000367399 ENST00000367397 ENST00000538660 ENST00000535699 ENST00000543483 ENST00000544212

Gene Information

This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa RP12 and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants some protein coding and some non-protein coding.[provided by RefSeq Apr 2012]

Gene Symbol:   CRB1
Gene Name:   crumbs homolog 1 (Drosophila)
Aliases:   LCA8, RP12
RefSeq:   NC_000001.10 NG_008483.1 NT_004487.19
Ensembl:   ENSG00000134376
Entrez:   23418
Chromosome Mapping:   1q31-q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.340000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download