PrimePCR™ SYBR® Green Assay: SRSF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039288

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SRSF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SRSF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036564
Assay Design:   exonic
Chromosome Location:   17:56084365-56084480question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000582730 ENST00000584773 ENST00000585096 ENST00000258962

Gene Information

This gene encodes a member of the arginine/serine-rich splicing factor protein family and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. In addition a pseudogene of this gene has been found on chromosome 13. [provided by RefSeq Sep 2010]

Gene Symbol:   SRSF1
Gene Name:   serine/arginine-rich splicing factor 1
Aliases:   ASF, FLJ53078, MGC5228, SF2, SF2p33, SFRS1, SRp30a
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000136450
Entrez:   6426
Chromosome Mapping:   17q22

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