PrimePCR™ SYBR® Green Assay: TBC1D7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039337

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TBC1D7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TBC1D7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036613
Assay Design:   exonic
Chromosome Location:   6:13321199-13321293question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000356436 ENST00000379300 ENST00000452989 ENST00000450347 ENST00000422136 ENST00000446018 ENST00000420456 ENST00000428109 ENST00000416436 ENST00000379291 ENST00000421203 ENST00000379307 ENST00000343141

Gene Information

TBC1D7 belongs to a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334) yeast Bub2 and CDC16 (MIM 603461) (Nakashima et al. 2007 [PubMed 17658474]).[supplied by OMIM Jul 2010]

Gene Symbol:   TBC1D7
Gene Name:   TBC1 domain family, member 7
Aliases:   DKFZp686N2317, FLJ32666, PIG51, TBC7, dJ257A7.3
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000145979
Entrez:   51256
Chromosome Mapping:   6p24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download