PrimePCR™ SYBR® Green Assay: HS6ST2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0039509


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PrimePCR™ PreAmp for SYBR® Green Assay: HS6ST2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: HS6ST2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0036785
Assay Design:		exonic
Chromosome Location:		X:131762716-131762841question
Amplicon Length:		96
Splice Variants Targeted:		ENST00000370837 ENST00000370836 ENST00000521489 ENST00000370833 ENST00000406696

Gene Information

Heparan sulfate proteoglycans are ubiquitous components of the cell surface extracellular matrix and basement membranes and interact with various ligands to influence cell growth differentiation adhesion and migration. This gene encodes a member of the heparan sulfate (HS) sulfotransferase gene family which catalyze the transfer of sulfate to HS. Different family members and isoforms are thought to synthesize heparan sulfates with tissue-specific structures and functions. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		HS6ST2
Gene Name:		heparan sulfate 6-O-sulfotransferase 2
Aliases:		MGC130022, MGC130023
RefSeq:		NC_000023.10 NG_012840.1 NT_011786.16
Ensembl:		ENSG00000171004
Entrez:		90161
Chromosome Mapping:		Xq26.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	36.030000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.