PrimePCR™ SYBR® Green Assay: ZHX3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0039548


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PrimePCR™ PreAmp for SYBR® Green Assay: ZHX3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ZHX3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0036824
Assay Design:		exonic
Chromosome Location:		20:39833432-39833556question
Amplicon Length:		95
Splice Variants Targeted:		ENST00000559234 ENST00000557816 ENST00000544979 ENST00000560361 ENST00000441102 ENST00000373261 ENST00000436440 ENST00000436099 ENST00000419740 ENST00000373263 ENST00000540170

Gene Information

This gene encodes a member of the zinc fingers and homeoboxes (ZHX) gene family. The encoded protein contains two C2H2-type zinc fingers and five homeodomains and forms a dimer with itself or with zinc fingers and homeoboxes family member 1. In the nucleus the dimerized protein interacts with the A subunit of the ubiquitous transcription factor nuclear factor-Y and may function as a transcriptional repressor. [provided by RefSeq Jul 2008]

Gene Symbol:		ZHX3
Gene Name:		zinc fingers and homeoboxes 3
Aliases:		KIAA0395, TIX1
RefSeq:		NC_000020.10 NT_011362.10
Ensembl:		ENSG00000174306
Entrez:		23051
Chromosome Mapping:		20q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.050000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.