PrimePCR™ SYBR® Green Assay: ANXA8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039845

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ANXA8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANXA8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037122
Assay Design:   exonic
Chromosome Location:   10:48270498-48270598question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000357718 ENST00000344416 ENST00000456111 ENST00000358140 ENST00000359178 ENST00000414655 ENST00000545298 ENST00000340243 ENST00000374277 ENST00000538825 ENST00000449464 ENST00000396328 ENST00000557913 ENST00000360509 ENST00000559418 ENST00000584982 ENST00000583874 ENST00000585281 ENST00000583911

Gene Information

This gene encodes a member of the annexin family of evolutionarily conserved Ca2+ and phospholipid binding proteins. The encoded protein may function as an an anticoagulant that indirectly inhibits the thromboplastin-specific complex. Overexpression of this gene has been associated with acute myelocytic leukemia. A highly similar duplicated copy of this gene is found in close proximity on the long arm of chromosome 10. [provided by RefSeq Jul 2008]

Gene Symbol:   ANXA8
Gene Name:   annexin A8
Aliases:   ANX8, FLJ53095
RefSeq:   NC_000010.10 NT_030772.10
Ensembl:   ENSG00000165390
Entrez:   653145
Chromosome Mapping:   10q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.250000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download