PrimePCR™ SYBR® Green Assay: RSPH10B2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039857

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RSPH10B2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RSPH10B2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037134
Assay Design:   exonic
Chromosome Location:   7:6803611-6803723question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000263314 ENST00000209875 ENST00000281187 ENST00000503271 ENST00000503423 ENST00000506722 ENST00000504454 ENST00000394537 ENST00000357077 ENST00000264366 ENST00000405415 ENST00000404406 ENST00000337579 ENST00000441023 ENST00000230671 ENST00000524041 ENST00000403107 ENST00000404077 ENST00000297186 ENST00000433859 ENST00000359718

Gene Information

Description Not Available

Gene Symbol:   RSPH10B2
Gene Name:   radial spoke head 10 homolog B2 (Chlamydomonas)
Aliases:   FLJ36092, FLJ54347, FLJ95901, RSPH10B
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000169402
Entrez:   728194
Chromosome Mapping:   7p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.720000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download