PrimePCR™ SYBR® Green Assay: POTEF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039975

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: POTEF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POTEF, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037252
Assay Design:   exonic
Chromosome Location:   2:130877568-130877710question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000343518 ENST00000358970 ENST00000407559 ENST00000301740 ENST00000576924 ENST00000571378 ENST00000314616 ENST00000347486 ENST00000409832 ENST00000454856 ENST00000438063 ENST00000439682 ENST00000551509 ENST00000299443 ENST00000409914 ENST00000357462 ENST00000360967 ENST00000361163 ENST00000451531 ENST00000409602 ENST00000327822 ENST00000356920 ENST00000358087

Gene Information

Description Not Available

Gene Symbol:   POTEF
Gene Name:   POTE ankyrin domain family, member F
Aliases:   A26C1B, POTE2alpha, POTEACTIN
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000196604
Entrez:   728378
Chromosome Mapping:   2q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.260000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download