PrimePCR™ SYBR® Green Assay: HSPA1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040035

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HSPA1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HSPA1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037312
Assay Design:   exonic
Chromosome Location:   6:31797418-31797533question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000372168 ENST00000361101 ENST00000589296 ENST00000446868 ENST00000591455 ENST00000589297 ENST00000585509 ENST00000558518 ENST00000557933 ENST00000455727 ENST00000535915 ENST00000558013 ENST00000252444 ENST00000400040 ENST00000383389 ENST00000548079 ENST00000391555 ENST00000400585 ENST00000400573 ENST00000262608 ENST00000342247 ENST00000407684 ENST00000375651 ENST00000458062 ENST00000375650 ENST00000545241 ENST00000430065 ENST00000422919 ENST00000549607 ENST00000450744 ENST00000313084 ENST

Gene Information

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   HSPA1B
Gene Name:   heat shock 70kDa protein 1B
Aliases:   FLJ54328, HSP70-1B, HSP70-2, HSPA1A
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167248.1
Ensembl:   ENSG00000204388
Entrez:   3304
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.670000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download