PrimePCR™ SYBR® Green Assay: SUN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040466

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SUN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SUN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037743
Assay Design:   exonic
Chromosome Location:   7:878476-881601question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000389574 ENST00000435699 ENST00000440380 ENST00000439679 ENST00000424128 ENST00000457598 ENST00000421580 ENST00000405266 ENST00000401592 ENST00000403868 ENST00000450881 ENST00000456758 ENST00000457378 ENST00000452783 ENST00000425407

Gene Information

This gene is a member of the unc-84 homolog family and encodes a nuclear nuclear envelope protein with an Unc84 (SUN) domain. The protein is involved in nuclear anchorage and migration. Alternatively spliced transcript variants have been described. [provided by RefSeq Jan 2010]

Gene Symbol:   SUN1
Gene Name:   Sad1 and UNC84 domain containing 1
Aliases:   FLJ12407, KIAA0810, MGC176649, UNC84A
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000164828
Entrez:   23353
Chromosome Mapping:   7p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.050000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download