PrimePCR™ SYBR® Green Assay: AP2S1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040561


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PrimePCR™ PreAmp for SYBR® Green Assay: AP2S1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: AP2S1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0037838
Assay Design:		exonic
Chromosome Location:		19:47349289-47354028question
Amplicon Length:		88
Splice Variants Targeted:		ENST00000352203 ENST00000601498 ENST00000599990 ENST00000263270 ENST00000597020 ENST00000601649

Gene Information

One of two major clathrin-associated adaptor complexes AP-2 is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains a medium chain and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts. [provided by RefSeq Jul 2008]

Gene Symbol:		AP2S1
Gene Name:		adaptor-related protein complex 2, sigma 1 subunit
Aliases:		AP17, AP17-DELTA, CLAPS2
RefSeq:		NC_000019.9 NT_011109.16
Ensembl:		ENSG00000042753
Entrez:		1175
Chromosome Mapping:		19q13.2-q13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.350000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.