PrimePCR™ SYBR® Green Assay: APOL4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040679

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: APOL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APOL4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037956
Assay Design:   exonic
Chromosome Location:   22:36600674-36600810question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000354903 ENST00000526177 ENST00000533784 ENST00000529721 ENST00000336898 ENST00000533805 ENST00000376282 ENST00000443276 ENST00000381232 ENST00000555818 ENST00000358550 ENST00000537413 ENST00000376325 ENST00000240662 ENST00000391761 ENST00000359649 ENST00000284648 ENST00000249075 ENST00000404685 ENST00000405511 ENST00000429038 ENST00000457630 ENST00000419360 ENST00000449084 ENST00000397275 ENST00000436763 ENST00000352371 ENST00000332987 ENST00000328429 ENST00000358502 ENST00000249066 ENST

Gene Information

The protein encoded by this gene is a member of the apolipoprotein L family and may play a role in lipid exchange and transport throughout the body as well as in reverse cholesterol transport from peripheral cells to the liver. Two transcript variants encoding two different isoforms have been found for this gene. Only one of the isoforms appears to be a secreted protein. [provided by RefSeq Jul 2008]

Gene Symbol:   APOL4
Gene Name:   apolipoprotein L, 4
Aliases:   APOL-IV, APOLIV
RefSeq:   NC_000022.10 NG_023437.1 NT_011520.12
Ensembl:   ENSG00000100336
Entrez:   80832
Chromosome Mapping:   22q11.2-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.260000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download